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  • Written by Don Arnold, Professor of Biological Sciences and Biomedical Engineering, USC Dornsife College of Letters, Arts and Sciences
Where are memories stored in the brain? New research suggests they may be in the connections between your brain cells

All memory storage devices, from your brain to the RAM in your computer, store information by changing their physical qualities. Over 130 years ago, pioneering neuroscientist Santiago Ramón y Cajal[1] first suggested that the brain stores information by rearranging the connections, or synapses, between neurons.

Since then, neuroscientists have attempted to understand the physical changes associated with memory formation. But visualizing and mapping synapses is challenging to do. For one, synapses are very small and tightly packed together. They’re roughly 10 billion times smaller[2] than the smallest object a standard clinical MRI can visualize. Furthermore, there are approximately 1 billion synapses[3] in the mouse brains researchers often use to study brain function, and they’re all the same opaque to translucent color as the tissue surrounding them.

Diagram showing structure of a chemical synapse and neurotransmitter release.
Synapses comprise the very end of the transmitting neuron, the very beginning of the receiving neuron, and the tiny gap between them. ttsz/iStock via Getty Images Plus[4]

A new imaging technique[5] my colleagues and I[6] developed, however, has allowed us to map synapses during memory formation. We found that the process of forming new memories changes how brain cells are connected to one another. While some areas of the brain create more connections, others lose them.

Mapping new memories in fish

Previously, researchers focused on recording the electrical signals[7] produced by neurons. While these studies have confirmed that neurons change their response to particular stimuli after a memory is formed, they couldn’t pinpoint what drives those changes.

To study how the brain physically changes when it forms a new memory, we created 3D maps of the synapses of zebrafish before and after memory formation. We chose zebrafish[8] as our test subjects because they are large enough to have brains that function like those of people, but small and transparent enough to offer a window into the living brain.

Black and white image of larval zebrafish. Zebrafish are particularly fitting models for neuroscience research. Zhuowei Du and Don B. Arnold, CC BY-NC-ND[9]

To induce a new memory in the fish, we used a type of learning process called classical conditioning[10]. This involves exposing an animal to two different types of stimuli simultaneously: a neutral one that doesn’t provoke a reaction and an unpleasant one that the animal tries to avoid. When these two stimuli are paired together enough times, the animal responds to the neutral stimulus as if it were the unpleasant stimulus, indicating that it has made an associative memory[11] tying these stimuli together.

As an unpleasant stimulus, we gently heated the fish’s head with an infrared laser. When the fish flicked its tail, we took that as an indication that it wanted to escape. When the fish is then exposed to a neutral stimulus, a light turning on, tail flicking meant that it’s recalling what happened when it previously encountered the unpleasant stimulus.

Diagram illustrating classical conditioning of a dog to salivate in response to a ringing bell. Pavlov’s dog is the most well-known example of classical conditioning, in which a dog salivates in response to a ringing bell because it has formed an associative memory between the bell and food. Lili Chin/Flickr, CC BY-NC-ND[12][13]

To create the maps, we genetically engineered zebrafish with neurons that produce fluorescent proteins that bind to synapses and make them visible. We then imaged the synapses with a custom-built microscope that uses a much lower dose of laser light than standard devices that also use fluorescence to generate images. Because our microscope caused less damage to the neurons, we were able to image the synapses without losing their structure and function.

Image of magenta-colored neurons in a live fish brain, with the synapses colored in green This image shows neurons in a live fish brain, with the synapses colored in green. Zhuowei Du and Don B. Arnold, CC BY-NC-ND[14]

When we compared the 3D synapse maps before and after memory formation, we found that neurons in one brain region, the anterolateral dorsal pallium, developed new synapses while neurons predominantly in a second region, the anteromedial dorsal pallium, lost synapses. This meant that new neurons were pairing together, while others destroyed their connections. Previous experiments have suggested that the dorsal pallium[15] of fish may be analogous to the amygdala of mammals, where fear memories are stored.

Surprisingly, changes in the strength of existing connections between neurons that occurred with memory formation were small and indistinguishable from changes in control fish that did not form new memories. This meant that forming an associative memory involves synapse formation and loss, but not necessarily changes in the strength of existing synapses, as previously thought.

Researchers from Howard Hughes Medical Institute captured video of the firing neurons of a baby zebrafish as it sees things and tries to move.

Could removing synapses remove memories?

Our new method of observing brain cell function could open the door not just to a deeper understanding of how memory actually works, but also to potential avenues for treatment of neuropsychiatric conditions like PTSD and addiction.

Associative memories[16] tend to be much stronger than other types of memories, such as conscious memories about what you had for lunch yesterday. Associative memories induced by classical conditioning, moreover, are thought to be analogous to traumatic memories that cause PTSD[17]. Otherwise harmless stimuli similar to what someone experienced at the time of the trauma can trigger recall of painful memories. For instance, a bright light or a loud noise could bring back memories of combat. Our study reveals the role that synaptic connections may play in memory, and could explain why associative memories can last longer and be remembered more vividly than other types of memories.

Currently the most common treatment for PTSD, exposure therapy[18], involves repeatedly exposing the patient to a harmless but triggering stimulus in order to suppress recall of the traumatic event. In theory, this indirectly remodels the synapses of the brain to make the memory less painful. Although there has been some success with exposure therapy, patients are prone to relapse[19]. This suggests that the underlying memory causing the traumatic response has not been eliminated.

Conceptually tied to classical conditioning, prolonged exposure therapy is one way to treat PTSD.

It’s still unknown whether synapse generation and loss actually drive memory formation. My laboratory[20] has developed technology that can quickly and precisely remove synapses[21] without damaging neurons. We plan to use similar methods to remove synapses in zebrafish or mice to see whether this alters associative memories.

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It might be possible to physically erase the associative memories that underlie devastating conditions like PTSD and addiction with these methods. Before such a treatment can even be contemplated, however, the synaptic changes encoding associative memories need to be more precisely defined. And there are obviously serious ethical and technical hurdles that would need to be addressed. Nevertheless, it’s tempting to imagine a distant future in which synaptic surgery could remove bad memories.

References

  1. ^ Santiago Ramón y Cajal (doi.org)
  2. ^ 10 billion times smaller (doi.org)
  3. ^ 1 billion synapses (doi.org)
  4. ^ ttsz/iStock via Getty Images Plus (www.gettyimages.com)
  5. ^ new imaging technique (dx.doi.org)
  6. ^ and I (scholar.google.com)
  7. ^ recording the electrical signals (doi.org)
  8. ^ zebrafish (dx.doi.org)
  9. ^ CC BY-NC-ND (creativecommons.org)
  10. ^ classical conditioning (blogs.scientificamerican.com)
  11. ^ associative memory (www.verywellmind.com)
  12. ^ Lili Chin/Flickr (www.flickr.com)
  13. ^ CC BY-NC-ND (creativecommons.org)
  14. ^ CC BY-NC-ND (creativecommons.org)
  15. ^ dorsal pallium (doi.org)
  16. ^ Associative memories (www.verywellmind.com)
  17. ^ traumatic memories that cause PTSD (doi.org)
  18. ^ exposure therapy (doi.org)
  19. ^ prone to relapse (doi.org)
  20. ^ My laboratory (dornsife.usc.edu)
  21. ^ remove synapses (doi.org)
  22. ^ Sign up today (memberservices.theconversation.com)

Authors: Don Arnold, Professor of Biological Sciences and Biomedical Engineering, USC Dornsife College of Letters, Arts and Sciences

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