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  • Written by Angie Hilliker, Associate Professor of Biology, University of Richmond
Customizing mRNA is easy, and that's what makes it the next frontier for personalized medicine − a molecular biologist explains

While using mRNA as medicine is new, mRNA has been inside you for your entire life. The cells in your body create mRNAs that serve as instructions to make specific proteins you need to function. Researchers can create new mRNAs to correct those instructions when they aren’t working.

I am a molecular biologist[1] who studies how cells control their mRNAs to make the proteins they need, a basic question of how life works at the cellular level. While most scientists studying mRNAs are not creating new drugs, this fundamental understanding of how mRNA works laid the foundation[2] for other scientists to create effective mRNA medicines like COVID-19 vaccines[3].

By tweaking these instructions, scientists can create powerful new medicines to repair a variety of problems in your cells.

What does mRNA do?

To understand what the mRNAs in your cells are doing for you, let’s start with its more well-known relative, DNA.

DNA is like a set of cookbooks full of different recipes, or genes, to make proteins. People make about 100,000 different proteins[4] that are essential for normal function[5], such as breaking down nutrients and carrying out other important chemical reactions.

When cells need to make one of those proteins, they don’t read the recipe directly from DNA. Instead, they make a copy in the form of a similar molecule – that’s the mRNA[6]. The “m” stands for messenger, as mRNA contains the message, or recipe, that codes for a protein. About one-third of a cell’s energy[7] is devoted to maintaining the proteins you need, so cells are well equipped to recognize, use and then destroy mRNA once it’s no longer needed.

Diagram illustrating flow of DNA transcription to RNA translation to protein
mRNA is the intermediary between DNA and protein. Rujirat Boonyong/iStock via Getty Images[8]

The language of mRNA is made of four building blocks called nucleotides[9], nicknamed A, U, C and G. The recipe to make a protein contains only three-letter words, meaning there are just 64 possible words. Scientists know exactly which words correspond to each protein building block, so they can easily read an mRNA recipe and know what protein will be made. Mutations in the DNA cookbook can alter or delete an mRNA recipe, leading to disease-causing mistakes in critical proteins.

Why do mRNAs make great medicine?

While mRNA has been within us all along, it took decades of research[10] for scientists to understand how cells recognize mRNA and use it to make protein. But it eventually became clear that mRNA could be a powerful medical tool.

Since scientists understand how mRNAs code for proteins, they can easily create recipes for any protein. These recipes can be edited to meet the needs[11] of the patient, whether this means providing a whole new mRNA recipe or tweaking an existing one to make a slight variation of the protein.

Producing mRNA treatments is also scalable[12] because scientists can make large amounts of mRNA in the lab. The method to make one mRNA is the same for all mRNAs, unlike typical drugs where each compound has its own unique chemistry and requires different manufacturing methods. It’s like learning how to make risotto: Once you’ve learned the basic recipe, you can make endless variations.

Another benefit of using mRNAs as drugs are cells’ natural ability to destroy them[13] when they aren’t needed. Since mRNAs aren’t permanent, doses can be easily changed to meet the changing needs of the patient.

mRNA vaccines beyond COVID-19

The COVID-19 vaccines[14] from Moderna and Pfizer-BioNTech are the first mRNA-based medicines to gain FDA approval. When these vaccines are injected into your arm, the mRNA is absorbed into some of your cells, which read the mRNA recipe and make the spike protein the virus uses to invade cells. Your immune system recognizes this spike protein as foreign and makes antibodies that prepare your body to attack the virus if you encounter it later.

These mRNA vaccines demonstrate the flexibility of mRNA-based therapies[15]. As the virus that causes COVID-19 mutates, new viral variants can evade existing antibodies and cause new waves of illness. However, scientists are able to sequence new mRNA recipes based on these variants and tweak the vaccine recipes to match them. Boosters containing these edited recipes teach your body to make new antibodies that target the latest versions of the viral spike protein.

The Pfizer-BioNTech and Moderna COVID-19 vaccines were the first to use mRNA for immunization.

There are already clinical trials underway for other mRNA-based vaccines, including vaccines for seasonal flu[16], herpes[17] and respiratory syncytial virus[18].

There are also many more vaccines in earlier stages of development to combat diseases like norovirus, Lyme disease[19], Zika[20] and shingles[21].

mRNA as treatment for disease

The potential for mRNA-based medicine extends beyond vaccines to prevent infectious disease. One example is the use of mRNA to treat cancer[22].

Some mRNA cancer treatments work like vaccines by training your immune system to specifically target cancer cells. As cancer cells grow, they rapidly gain mutations in many genes. Cancer vaccines contain mRNA recipes based on mutations commonly found in certain types of tumors. When injected into the body, the mRNAs from the vaccines allow normal cells to make those mutated proteins and broadcast them to the immune system, ramping up production of antibodies. These antibodies bind to cancer cells and mark them for immune attack.

Finding the correct protein target for a given cancer[23] is essential. Ideally, the target is unique to the cancer cell so the immune system doesn’t attack healthy cells. The target protein should also be easy for the immune system to sense, making surface proteins good targets. Cancer vaccines, like BioNTech’s BNT-111[24] for melanoma, target the most common cancer mutations in hope of helping many patients. But patients won’t benefit from the treatment if their cancer cells don’t have those particular mutations.

Because it is so easy to change the mRNA recipes, cancer vaccines can be part of a personalized medicine plan[25] where doctors sample a patient’s tumor, sequence key genes and adjust the mRNA treatment to include recipes specific to that patient’s cancer. Clinical trials using this personalized approach for pancreatic cancer[26] are underway.

The future of mRNA-based medicine

Many diseases arise from cells making the wrong protein, a mutant version of protein or too little of the normal protein. If scientists can deliver a corrected version of the mRNA recipe to enough affected cells, then the mRNA will provide the means to make the proper protein.

Scientists are exploring the use of mRNA to treat heart disease[27], neurodegenerative disease[28], bone loss[29] and much more. Although most of these studies are still very early in development, they provide hope for future treatments using mRNA for protein replacement therapies.

For example, one mRNA drug increases the formation of new blood vessels[30], which can improve wound healing in diabetic patients[31] who have poor blood circulation and higher amputation risks. Another example is using mRNAs to treat propionic acidemia[32], a disease where children have low levels of two liver proteins that normally prevent toxic byproducts from building up in the body.

The ability to easily customize and produce mRNA increases their potential as effective, personalized therapies – with fewer side effects – that can help many people.

References

  1. ^ molecular biologist (scholar.google.com)
  2. ^ laid the foundation (theconversation.com)
  3. ^ COVID-19 vaccines (theconversation.com)
  4. ^ about 100,000 different proteins (www.broadinstitute.org)
  5. ^ normal function (theconversation.com)
  6. ^ the mRNA (www.genome.gov)
  7. ^ one-third of a cell’s energy (doi.org)
  8. ^ Rujirat Boonyong/iStock via Getty Images (www.gettyimages.com)
  9. ^ building blocks called nucleotides (www.genome.gov)
  10. ^ decades of research (doi.org)
  11. ^ edited to meet the needs (doi.org)
  12. ^ is also scalable (doi.org)
  13. ^ natural ability to destroy them (doi.org)
  14. ^ COVID-19 vaccines (theconversation.com)
  15. ^ flexibility of mRNA-based therapies (theconversation.com)
  16. ^ seasonal flu (theconversation.com)
  17. ^ herpes (theconversation.com)
  18. ^ respiratory syncytial virus (theconversation.com)
  19. ^ norovirus, Lyme disease (www.forbes.com)
  20. ^ Zika (doi.org)
  21. ^ shingles (www.pfizerclinicaltrials.com)
  22. ^ use of mRNA to treat cancer (theconversation.com)
  23. ^ correct protein target for a given cancer (theconversation.com)
  24. ^ BioNTech’s BNT-111 (doi.org)
  25. ^ personalized medicine plan (www.genome.gov)
  26. ^ personalized approach for pancreatic cancer (www.mskcc.org)
  27. ^ heart disease (spectrum.ieee.org)
  28. ^ neurodegenerative disease (theconversation.com)
  29. ^ bone loss (doi.org)
  30. ^ formation of new blood vessels (www.nature.com)
  31. ^ wound healing in diabetic patients (www.healthline.com)
  32. ^ propionic acidemia (clinicaltrials.gov)

Authors: Angie Hilliker, Associate Professor of Biology, University of Richmond

Read more https://theconversation.com/customizing-mrna-is-easy-and-thats-what-makes-it-the-next-frontier-for-personalized-medicine-a-molecular-biologist-explains-216127

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