Gain-of-function research is more than just tweaking risky viruses – it's a routine and essential tool in all biology research
- Written by Seema Lakdawala, Associate Professor of Microbiology and Immunology at Emory University and Adjunct Professor Microbiology and Molecular Genetics, University of Pittsburgh
The term “gain of function” is often taken to refer to research with viruses that puts society at risk of an infectious disease outbreak for questionable gain. Some research on emerging viruses can result in variants that gain the ability to infect people but this does not necessarily mean the research is dangerous or that it is not fruitful. Concerns have focused on lab research on the virus that causes bird flu[1] in 2012 and on the virus that causes COVID-19[2] since 2020. The National Institutes of Health had previously implemented a three-year moratorium[3] on gain-of-function research on certain viruses, and some U.S. legislatures have proposed bills prohibiting[4] gain-of-function research on “potentially pandemic pathogens.”
The possibility that a genetically modified virus could escape the lab needs to be taken seriously. But it does not mean that gain-of-function experiments are inherently risky or the purview of mad scientists. In fact, gain-of-function approaches are a fundamental tool in biology used to study much more than just viruses, contributing to many, if not most, modern discoveries in the field, including penicillin[5], cancer immunotherapies[6] and drought-resistant crops[7].
As scientists who[8] study viruses[9], we believe that misunderstanding the term “gain of function” as something nefarious comes at the cost of progress in human health, ecological sustainability and technological advancement. Clarifying what gain-of-function research really is can help clarify why it is an essential scientific tool.
What is gain of function?
To study how a living thing operates, scientists can change a specific part of it and then observe the effects. These changes sometimes result in the organism’s gaining a function it didn’t have before or losing a function it once had.
For example, if the goal is to enhance the tumor-killing ability of immune cells, researchers can take a sample of a person’s immune cells and modify them to express a protein that specifically targets cancer cells. This mutated immune cell, called a CAR-T cell[10] thereby “gains the function” of being able to bind to cancerous cells and kill them. The advance of similar immunotherapies that help the immune system attack cancer cells is based on the exploratory research of scientists who synthesized such “Frankenstein” proteins[11] in the 1980s. At that time, there was no way to know how useful these chimeric proteins would be to cancer treatment today, some 40 years later.
CAR-T cell therapy involves giving a patient’s immune cells an increased ability to target cancer cells.Similarly, by adding specific genes into rice, corn or wheat plants that increase their production in diverse climates, scientists have been able to produce plants that are able to grow and thrive in geographical regions they previously could not. This is a critical advance to maintain food supplies in the face of climate change. Well-known examples of food sources that have their origins in gain-of-function research include rice plants[12] that can grow in high flood plains or in drought conditions or that contain vitamin A to reduce malnutrition.
Medical advances from gain-of-function research
Gain-of-function experiments are ingrained in the scientific process. In many instances, the benefits that stem from gain-of-function experiments are not immediately clear. Only decades later does the research bring a new treatment to the clinic or a new technology within reach.
The development of most antibiotics have relied on the manipulation of bacteria or mold[13] in gain-of-function experiments. Alexander Fleming’s initial discovery that the mold Penicillium rubens could produce a compound toxic to bacteria was a profound medical advance. But it wasn’t until scientists experimented with growth conditions and mold strains[14] that therapeutic use of penicillin became feasible. Using a specific growth medium allowed the mold to gain the function of increased penicillin production, which was essential for its mass production and widespread use as a drug.
Research on antibiotic resistance[16] also relies heavily on gain-of-function approaches. Studying how bacteria gain resistance[17] against drugs is essential to developing new treatments microbes are unable to evade quickly.
Gain-of-function research in virology has also been critical to the advancement of science and health. Oncolytic viruses[18] are genetically modified in the laboratory to infect and kill cancerous cells like melanoma. Similarly, the Johnson & Johnson COVID-19 vaccine[19] contains an adenovirus altered to produce the spike protein that helps the COVID-19 virus infect cells. Scientists developed live attenuated flu vaccines[20] by adapting them to grow at low temperatures and thereby lose the ability to grow at human lung temperatures.
By giving viruses new functions, scientists were able to develop new tools to treat and prevent disease.
Nature’s gain-of-function experiments
Gain-of-function approaches are needed to advance understanding of viruses in part because these processes already occur in nature.
Many viruses that infect such nonhuman animals as bats, pigs, birds and mice have the potential to spill over into people[21]. Every time a virus copies its genome, it makes mistakes. Most of these mutations are detrimental – they reduce a virus’s ability to replicate – but some may allow a virus to replicate faster or better in human cells. Variant viruses with these rare, beneficial mutations will spread better than other variants and therefore come to dominate the viral population – that is how natural selection works[22].
If these viruses can replicate even a little bit within people, they have the potential to adapt and thereby thrive in their new human hosts. That is nature’s gain-of-function experiment, and it is[23] happening constantly[24].
Gain-of-function experiments in the lab can help scientists anticipate the changes[25] viruses may undergo in nature by understanding what specific features allow them to transmit between people and infect them. In contrast to nature’s experiments, these are conducted in highly controlled lab conditions[26] designed to limit infection risk to laboratory personnel and others, including air flow control, personal protective equipment and waste sterilization.
Researchers and public health officials are concerned that the bird flu virus is evolving to more readily infect people.
Guadalupe Pardo/AP Photo[27]
It is important that researchers carefully observe lab safety to minimize the theoretical risk of infecting the general population. It is equally important that virologists continue to apply the tools of modern science to gauge the risk of natural viral spillovers before they become outbreaks.
A bird flu outbreak[28] is currently raging across multiple continents. While the H5N1 virus is primarily infecting birds, some people have gotten sick too. More spillover events can change the virus in ways that would allow it to transmit more efficiently among people[29], potentially leading to a pandemic.
Scientists have a better appreciation of the tangible risk of bird flu spillover because of gain-of-function experiments[30] published a decade ago[31]. Those lab studies showed that bird flu viruses could be transmitted through the air between ferrets within a few feet of one another. They also revealed multiple features of the evolutionary path the H5N1 virus would need to take before it becomes transmissible in mammals, informing what signatures researchers need to look out for during surveillance of the current outbreak.
Oversight on gain of function
Perhaps this sounds like a semantic argument, and in many respects it is. Many researchers[32] would likely agree that gain of function as a general tool is an important way to study biology that should not be restricted, while also arguing that it should be curtailed for research on specific dangerous pathogens. The problem with this argument is that pathogen research needs to include gain-of-function approaches in order to be effective – just as in any area of biology.
Oversight of gain-of-function research[33] on potential pandemic pathogens already exists. Multiple layers of safety measures at the institutional and national levels minimize the risks of virus research.
While updates to current oversight are not unreasonable, we believe that blanket bans or additional restrictions[34] on gain-of-function research do not make society safer. They may instead slow research in areas ranging from cancer therapies to agriculture. Clarifying which specific research areas are of concern regarding gain-of-function approaches can help identify how the current oversight framework can be improved.
References
- ^ virus that causes bird flu (www.theguardian.com)
- ^ virus that causes COVID-19 (theconversation.com)
- ^ three-year moratorium (www.science.org)
- ^ proposed bills prohibiting (www.washingtonexaminer.com)
- ^ penicillin (doi.org)
- ^ cancer immunotherapies (theconversation.com)
- ^ drought-resistant crops (www.sciencedaily.com)
- ^ scientists who (scholar.google.com)
- ^ study viruses (scholar.google.com)
- ^ CAR-T cell (theconversation.com)
- ^ Frankenstein” proteins (doi.org)
- ^ include rice plants (www.sciencenews.org)
- ^ manipulation of bacteria or mold (doi.org)
- ^ growth conditions and mold strains (www.sciencemuseum.org.uk)
- ^ Wesley/Stringer/Hulton Archive via Getty Images (www.gettyimages.com)
- ^ antibiotic resistance (doi.org)
- ^ gain resistance (theconversation.com)
- ^ Oncolytic viruses (www.cancer.gov)
- ^ Johnson & Johnson COVID-19 vaccine (www.cdc.gov)
- ^ live attenuated flu vaccines (onlinelibrary.wiley.com)
- ^ spill over into people (theconversation.com)
- ^ how natural selection works (www.amnh.org)
- ^ it is (doi.org)
- ^ happening constantly (doi.org)
- ^ anticipate the changes (doi.org)
- ^ highly controlled lab conditions (www.cdc.gov)
- ^ Guadalupe Pardo/AP Photo (newsroom.ap.org)
- ^ bird flu outbreak (theconversation.com)
- ^ transmit more efficiently among people (doi.org)
- ^ gain-of-function experiments (doi.org)
- ^ published a decade ago (doi.org)
- ^ Many researchers (www.statnews.com)
- ^ Oversight of gain-of-function research (doi.org)
- ^ blanket bans or additional restrictions (www.nih.gov)
Authors: Seema Lakdawala, Associate Professor of Microbiology and Immunology at Emory University and Adjunct Professor Microbiology and Molecular Genetics, University of Pittsburgh
